FastQCFastQC Report
Fri 5 May 2017
G150-M17-Index-18_GTCCGC-_BCALRAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M17-Index-18_GTCCGC-_BCALRAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21626067
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG10000.033.00617212
AGCGTCG10850.030.4205110
GCGTCGT11100.029.3388911
GATCGGA14950.022.3684561
GTCGCCG11050.021.90333644
GTCGTGT16400.020.66230813
ATCGGAA16700.020.288032
TCGTGTA16700.020.15927314
GAGCGTC18400.018.4162359
CGTGTAG19850.017.2926715
GATCTCG15850.017.20932434
TGGTCGC14400.016.49927142
AGAGCGT21950.015.9386848
AAGAGCG23600.014.7311617
GTGGTCG16650.014.66483241
TCGGAAG25750.014.1838993
ATCTCGG19750.014.03362335
GGTCGCC18800.012.87346943
CGGAAGA28750.012.16822054
TCTCGGT23950.011.29691636
Produced by FastQC (version 0.11.2)