FastQCFastQC Report
Fri 5 May 2017
G150-M18-Index-19_GTGAAA-_BCALRAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M18-Index-19_GTGAAA-_BCALRAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23916801
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA12450.022.4796241
AACGATC12050.021.51344538
CTCGTAT13300.021.14580344
ATCGGAA15300.018.2793982
TCTCGTA14850.018.19731543
GATCTCG14700.017.63465741
ACGATCT15350.017.03117239
CGATCTC15900.016.58007440
CGTCTGA18500.015.4726216
TGAAACG18850.014.92122535
AAACGAT18650.014.60799337
GAAACGA20100.014.43018536
ATCTCGT18650.014.13526142
ACACGTC21400.013.37566813
AGTCACG21950.012.91265728
GTCACGT23800.012.37161529
ACGTCTG23700.012.26267115
TCGGAAG24250.012.25910953
CGGAAGA26900.011.1339664
CACGTCT29400.010.40962714
Produced by FastQC (version 0.11.2)