FastQCFastQC Report
Fri 5 May 2017
G150-M18-Index-19_GTGAAA-_BCALRAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M18-Index-19_GTGAAA-_BCALRAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23916801
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG9400.029.26073512
GCGTCGT9850.028.8175811
AGCGTCG9800.028.06647910
GTCGCCG10800.021.39173144
GATCGGA13100.021.3292941
GAGCGTC15400.018.431839
GTCGTGT15100.018.36093913
ATCGGAA15900.017.9883082
TGGTCGC13650.017.56752842
TCGTGTA16550.016.88519514
GATCTCG15800.016.7065434
CGTGTAG18600.015.61563115
GTGGTCG15650.015.03969641
AGAGCGT20450.013.5573238
GGTCGCC18150.013.33457643
ATCTCGG21550.013.16732335
TCGGAAG22500.013.0061453
AAGAGCG25000.011.529997
TCGGTGG23250.011.44748938
CGGAAGA27250.011.2235114
Produced by FastQC (version 0.11.2)