FastQCFastQC Report
Fri 5 May 2017
G150-M18_thru_21.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M18_thru_21.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92642321
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG36650.028.0381912
GCGTCGT38350.027.13967911
AGCGTCG39050.026.31508410
GTCGCCG40350.020.72130844
GATCGGA51750.020.4491251
ATCGGAA61950.017.1177142
TCGTGTA63700.016.33895514
GTCGTGT64350.016.27658813
GAGCGTC65300.016.1071519
GATCTCG62200.015.10085534
TGGTCGC54750.014.94746942
CGTGTAG75700.014.24297415
GTGGTCG65900.012.81779841
AGAGCGT83000.012.7781658
GGTCGCC67550.012.76788643
ATCTCGG78850.012.5536235
TCGGAAG92350.011.8887833
AAGAGCG95800.011.4843387
CGGAAGA108700.010.2422744
TCGGTGG94500.010.21844538
Produced by FastQC (version 0.11.2)