FastQCFastQC Report
Fri 5 May 2017
G150-M20-Index-21_GTTTCG-_BCALRAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M20-Index-21_GTTTCG-_BCALRAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22668433
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG7900.029.76267633
GATCGGA11850.019.89861
TCTCGTA11000.019.37294443
CTCGTAT12050.018.96270444
TCGGAAT13000.017.40753637
GTTTCGG14050.017.20412434
ATCGGAA13950.016.7331032
CGGAATC13750.016.61631838
TTTCGGA14700.016.14400135
AATCTCG13900.015.17208441
CGTCTGA16800.014.94180216
ATCTCGT15150.014.50014642
ACACGTC17950.013.98424813
ACGTCTG19150.012.99219415
TTCGGAA18900.012.78857736
GTCACGT20450.012.14164329
ACGTTTC22100.011.03682232
AGTCACG23150.010.81942128
TCGGAAG22200.010.7127083
CGGAAGA24200.09.9191014
Produced by FastQC (version 0.11.2)