FastQCFastQC Report
Fri 5 May 2017
G150-M20-Index-21_GTTTCG-_BCALRAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M20-Index-21_GTTTCG-_BCALRAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22668433
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG8100.029.61100810
CGTCGTG8100.029.06768812
GCGTCGT8350.027.93387211
GTCGCCG8200.022.27128644
GATCGGA11250.020.5341911
ATCGGAA13500.016.7859232
GTCGTGT14700.016.16647313
GAGCGTC14850.016.1512099
TCGTGTA15450.015.80889114
GATCTCG14000.014.76940534
TGGTCGC12600.014.66608142
AGAGCGT17600.014.0024388
CGTGTAG17350.013.95077115
GTGGTCG14900.012.84396441
AAGAGCG20300.011.9233377
GGTCGCC15000.011.8810243
ATCTCGG18550.011.7394535
TCGGAAG20900.011.2644223
TCTCGGT21200.010.79078436
TCGGTGG22150.010.52649638
Produced by FastQC (version 0.11.2)