FastQCFastQC Report
Fri 5 May 2017
G150-M21-Index-22_CGTACG-_BCALRAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M21-Index-22_CGTACG-_BCALRAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24399864
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG8700.023.77505910
CGTCGTG8350.023.71750812
GCGTCGT8750.023.38771811
GATCGGA11800.018.4583261
GTCGCCG9650.017.55697644
ATCGGAA14350.015.7916032
TGGTCGC11100.013.87331842
TCGTGTA15250.013.5633114
GTCGTGT15750.012.85330813
GAGCGTC17650.012.2176629
GTGGTCG13600.012.13084841
CGTGTAG17450.012.10549515
GATCTCG15550.011.882634
AGAGCGT19600.011.3387688
GGTCGCC15050.011.1105143
TCGGAAG24700.09.3533483
ATCTCGG19750.09.24419635
AAGAGCG24800.08.961217
TCGGTGG24650.07.852626338
TCTCGGT23750.07.779847636
Produced by FastQC (version 0.11.2)