FastQCFastQC Report
Wed 24 May 2017
G152-M11-Index-13_AGTCAA-_ACAYD3ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M11-Index-13_AGTCAA-_ACAYD3ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16943811
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201450.11889296923814838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA21350.017.10406927
ATTGCGA22300.016.07981337
AATTGCG22750.016.05184436
CGAATGT24650.015.3485533
CTCGAAT30400.015.3255971
ATCCGAA22700.015.11733225
TAAGGCG46850.014.508389
CACCGGT25350.014.49159526
TGCGATA25050.014.4893939
CCGGTAG26150.014.38512528
AATCCGA23150.014.25325724
TTGCGAT25400.014.20392438
TCGGTTT33050.013.9101152
AAATCCG24000.013.83921123
CGGTAGG29450.013.07199829
GACACCG28450.013.06707324
GTCGGTT31650.012.9762081
GCGATAA28350.012.88156540
ACACCGG30150.012.54924425
CGGGGAA28100.012.41535951
Produced by FastQC (version 0.11.2)