FastQCFastQC Report
Wed 24 May 2017
G152-M11-Index-13_AGTCAA-_ACAYD3ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M11-Index-13_AGTCAA-_ACAYD3ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16943811
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN313710.185147249340777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC22250.016.9057751
CGATCAC20600.016.6607573
ACGATCA23300.015.1077612
CCACTAG34050.010.8549933
CGCGAAC2453.8063026E-610.7741771
TTAGATC23050.09.8311052
TAGATCC21800.09.587483
CGCTAGT7700.09.14433525
CGCAATT21250.08.9024281
CGACACT12550.08.7638551
TAGCCGT12550.08.7609434
CGCGGAT10550.08.7572111
TCGCTAG7850.08.68930224
TAGCCTC61950.08.5942932
GACGATC11100.08.5227852
CACGATA24900.08.49835442
AATCGAA11950.08.4691325
TACCGGT20200.08.38465210
CGATACA25000.08.37571744
TTAACGA32150.08.34730921
Produced by FastQC (version 0.11.2)