FastQCFastQC Report
Wed 24 May 2017
G152-M12-Index-14_AGTTCC-_ACAYD3ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M12-Index-14_AGTTCC-_ACAYD3ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16128600
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293080.1817144699477946No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT195990.12151705665711841No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT187830.11645772106692459No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC175760.10897412050642959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA22450.016.8541227
CTCGAAT28200.016.5989511
CGAATGT24050.016.0062163
TCGGTTT33250.015.9437722
AATTGCG24250.015.240087536
GTCGGTT33200.015.2296991
ATTGCGA24900.015.10734337
CCGGTAG25500.014.92450328
CACCGGT25600.014.86592926
CGGGATA10900.014.5847831
TGCGATA26250.014.49750539
TTGCGAT26900.014.31123338
CGGGCTA8100.014.1746481
CGGTTTG34650.013.7124863
TAAGGCG43900.013.6793419
TCGTTCG2451.540684E-913.46849628
CGGTAGG28850.013.34400629
ATCCGAA22250.013.14932425
GACACCG29100.013.07748524
AATCCGA22650.013.01390624
Produced by FastQC (version 0.11.2)