FastQCFastQC Report
Wed 24 May 2017
G152-M13-Index-15_ATGTCA-_ACAYD3ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M13-Index-15_ATGTCA-_ACAYD3ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21706646
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT341450.15730205394237323No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT301620.13895283499809227No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC250470.11538862337368934No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC240180.1106481397448505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA16750.018.0616441
CGAATGT29550.016.3766353
ACCGGTA25150.015.219933527
CTCGAAT34750.015.1242431
AATTGCG27150.014.42217236
ATTGCGA28050.014.27318837
TAAGGCG53350.014.2247999
TTGCGAT28850.013.4961538
CGGCGAT8100.013.3586491
TGCGATA29650.012.98302339
GTCGGAT11650.012.8894521
TCGGTTT38950.012.6502762
CACCGGT31850.012.36323326
AGGCGTC63400.012.00453511
CCGGTAG33650.011.70225128
GACACCG34650.011.68159324
GTCGGTT41600.011.6252491
AGGCTAA74750.011.623865
CGGGGAT25500.011.6042241
AAGGCGT68200.011.51450510
Produced by FastQC (version 0.11.2)