FastQCFastQC Report
Wed 24 May 2017
G152-M14-Index-7_CAGATC-_ACAYD3ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M14-Index-7_CAGATC-_ACAYD3ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15617230
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN294970.18887472362256302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC24800.020.0465451
ACGATCA25100.019.3714242
CGATCAC27350.017.2148673
CGACGAT4400.012.4988531
TAGCTCG24500.011.314486522
TAGCCGT12000.010.445436534
CCACTAG29350.010.11982253
ACGCAAC9150.09.85596910
CGAAAAT31050.09.8477271
AATGTAG66400.09.8080055
AGCTCGT28600.09.76975223
CGACAAT9750.09.70167351
TTAGATC18400.09.6852362
CACGCGA6950.09.4959299
TAATGTA65800.09.4625414
GCTCGTC30000.09.46190724
GGCAACT104950.09.3902761
TAGCCTC55650.09.3301762
ACGCGAG6300.09.07756510
TCTACGG43200.09.01355657
Produced by FastQC (version 0.11.2)