FastQCFastQC Report
Wed 24 May 2017
G152-M16-Index-16_CCGTCC-_ACAYD3ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M16-Index-16_CCGTCC-_ACAYD3ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15034093
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT224490.14932061415344444No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT209780.1395361861869552No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC190800.12691154697526483No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC161500.10742250962528968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA10650.018.0336151
TAAGGCG28550.014.4081269
CGAATGT24350.014.0923423
CTCGAAT26500.013.9118131
AATCCGA21400.013.67184724
ATCCGAA21600.013.44341325
CGGGCAT18800.013.3862871
GTCGGTT27000.013.327141
AAATCCG23150.013.01735723
ACCGGTA19550.012.71543127
CGGGGAT16500.012.7102121
TCGGTTT28500.012.580572
TACGTCA74300.012.22912432
CGGTTTG29500.012.154113
TGCTGCG26400.012.08184211
ACGTCAC77050.011.96405433
ATACGTC76200.011.89512831
CGGGCGT5300.011.6625981
GTCGGAT8600.011.5512021
ATCATGC31800.011.48308857
Produced by FastQC (version 0.11.2)