FastQCFastQC Report
Wed 24 May 2017
G152-M4-Index-9_GATCAG-_ACAYD3ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M4-Index-9_GATCAG-_ACAYD3ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15848955
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN295980.18675048291827442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTAG37150.012.55479053
CGATCAC7150.011.0753071
CGACGAT6300.010.8238171
ACGCTAA8350.010.54083326
TTAGATC24100.010.1327182
TAGCCTC67800.09.8966942
CGTAATC4907.2759576E-129.87786723
TTAATCG4501.5279511E-109.77945829
TAGATCC22450.09.6037793
CACTAGC48950.09.4383844
CCTAACG9000.09.28886122
TAACGCG3106.159533E-69.2259854
CTTAGAT25900.09.1724061
CTAGCCT79100.08.9266371
CGCCGAT4451.2900273E-88.8975781
TACCGGT17950.08.82305610
CGACAAT10300.08.7560031
ACTAGCC53850.08.7024225
CGCAATT20550.08.6702681
CTAACGC9900.08.66694423
Produced by FastQC (version 0.11.2)