FastQCFastQC Report
Wed 24 May 2017
G152-M8-Index-12_CTTGTA-_ACAYD3ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M8-Index-12_CTTGTA-_ACAYD3ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16111499
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN301550.18716445937153334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC12750.012.2510741
TTAGATC20800.011.9534732
CCACTAG32850.011.2523543
TAGATCC21900.011.1518863
CGACGAT4850.010.4330711
TAGCCTC63250.010.3665612
ACGCAAC10400.010.15221110
CACGATC15800.010.02539351
CGCAATT18050.09.7507681
TCATACG30550.09.720201510
TACGCAA10350.09.5652329
CTTAGAT27500.09.4400731
CTAGCCT73000.09.432951
ACAATCG5850.09.4027223
ACGATCA16350.09.1510342
CGACAAT10750.09.0047221
ATACGAT33600.08.96712
ATCGCTC18100.08.88819542
AGATCCA88000.08.8509544
CGTACTT17500.08.80127826
Produced by FastQC (version 0.11.2)