FastQCFastQC Report
Wed 24 May 2017
G152-M9-Index-5_ACAGTG-_ACAYD3ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG152-M9-Index-5_ACAGTG-_ACAYD3ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15845711
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN291140.18373426096184642No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA172150.10864138567212289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC30450.025.5748061
ACGATCA30650.024.6937142
CGATCAC38250.019.3845443
TCACAAG69450.011.5621256
GGCAACT116950.010.6090181
CCACTAG29600.010.33193
TTAGATC19350.010.1196982
TAACGCG2659.694235E-69.9621357
TTAGACT110300.09.9527278
ACTTTAG115700.09.9077865
GCAACTT117650.09.8367642
CGCAATT18350.09.8304671
TACGCAA9400.09.8293799
TAATGTA66250.09.76376154
TAGCCTC57350.09.7061182
TAGACTT112650.09.6667099
AATGTAG70050.09.5799675
TTTAGAC115300.09.5402077
ATCCATG117750.09.380980528
CTTTAGA119400.09.3047386
Produced by FastQC (version 0.11.2)