FastQCFastQC Report
Wed 24 May 2017
G153-M1-Index-18_GTCCGC-_ACAYD3ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M1-Index-18_GTCCGC-_ACAYD3ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14201098
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162120.1141601867686569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGT23550.015.4113823
CTCGAAT27100.014.7561641
ATCCGAA18550.013.75670925
GTCGGTT25800.013.101951
AATCCGA19300.012.99406324
AAATCCG20250.012.38429223
TAAGGCG39050.012.0546619
TCGAATG28650.012.0537872
TCGGTTT27950.012.0408772
GTCGGAT8700.011.4276641
CGGGGAA26150.011.4058131
TACGTCA13550.011.36565332
CGCGTGT4750.011.1630231
ACCGGTA16800.011.13034527
TCCGCCC35800.011.06084929
CCTACGA16800.010.9978520
AGTTCCG35650.010.92222726
CGGGGAT17000.010.916781
GCGCATT35050.010.86001644
CTCGTTT20150.010.8548621
Produced by FastQC (version 0.11.2)