FastQCFastQC Report
Wed 24 May 2017
G153-M1-Index-18_GTCCGC-_ACAYD3ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M1-Index-18_GTCCGC-_ACAYD3ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14201098
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN299850.21114564521701068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC11950.011.9678483
CGACGAT5750.011.47798251
CCACTAG35050.011.3621663
TTAGATC21750.010.9245322
TAGATCC21050.010.4524593
ATCGCTC17550.010.3031542
TAGCCTC69050.010.227742
CACGATC16500.010.1330881
CTAGCCT76850.09.7616371
CTTAGAT26700.09.722611
TCTTCGT70550.09.37722944
TACGCAA10550.09.1744519
ACGATCA16550.09.1725352
GGGTAAC73300.09.09375223
CGAAAAT23300.09.0641581
CACTAGC45800.08.9847344
CACGATA23350.08.87716542
CTCTTCG74450.08.82664643
CGCAATT18450.08.8236341
CCGGAAT30050.08.7851451
Produced by FastQC (version 0.11.2)