FastQCFastQC Report
Wed 24 May 2017
G153-M10-Index-23_GAGTGG-_ACAYD3ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M10-Index-23_GAGTGG-_ACAYD3ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15384496
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC237170.15416169629476326No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT236160.15350519120028372No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC183320.11915892467325546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA10100.018.1622371
CGAATGT24500.017.6863613
CTCGAAT28100.016.9886971
ACCGGTA17050.016.51571327
ATCCGAA19750.015.59449425
AATTGCG18650.015.21479636
AATCCGA20050.015.14156724
ATTGCGA19300.014.930325537
GTCGGTT27850.014.9191911
TCGGTTT28050.014.8987762
CACCGGT19900.014.37145126
CGGCGAT4950.014.28753951
AAATCCG21050.014.10817323
TTGCGAT20850.013.82035238
GCGCATT30850.013.7645944
TACGTCA85850.013.7616732
TCGACTA4750.013.42993112
TGCGATA21200.013.38410639
GTCGGAT9250.013.3800871
ATACGTC88200.013.34485531
Produced by FastQC (version 0.11.2)