FastQCFastQC Report
Wed 24 May 2017
G153-M10-Index-23_GAGTGG-_ACAYD3ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M10-Index-23_GAGTGG-_ACAYD3ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15384496
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN369370.24009236311673782No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT212710.13826257291756583No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT157850.10260329620157853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC40050.029.5508791
ACGATCA42350.027.3243562
CGATCAC50500.022.916313
TCACAAG81350.014.6567626
TAATGTA73550.012.1471194
GATCACA104200.012.0164014
AATGTAG78800.011.9228585
ATGTAGA89900.010.4242826
CTTTAAT101900.010.2328071
TTAGATC19200.010.1977982
GTAGAAA80250.010.1700988
CACAAGA127900.09.9243387
TAGATCC22450.09.898183
ATCGCTC14050.09.88979842
TTAATGT93650.09.8201663
TAATGGG79050.09.79003419
CGAAAAT30150.09.55816651
GTTGATT25850.09.5312111
GGCAACT120000.09.4409691
TAGACTT109700.09.3246839
Produced by FastQC (version 0.11.2)