FastQCFastQC Report
Wed 24 May 2017
G153-M11-Index-5_ACAGTG-_ACAYD3ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M11-Index-5_ACAGTG-_ACAYD3ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15007145
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT272030.18126698982384723No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC256100.1706520460753861No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC253560.16895951894914055No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT217320.14481102168333815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA12600.019.818811
CTCGAAT27500.016.4736861
CGAATGT23750.015.0960933
CGCGTGT3700.014.9316541
TACGTCA86750.014.63262232
ACCGGTA20950.014.49054927
CGGGGAT17550.014.3548081
ATACGTC89350.014.23116331
AATCCGA18800.014.04148924
TCGGTTT29350.013.9393492
GTCGGTT30700.013.74879551
ACGTCAC92250.013.73636733
CATACGT96550.013.51114630
TCATACG96800.013.49897729
ATCCGAA19500.013.424623525
ATTGCGA22850.013.1890237
CGTCACA97000.013.130734
AATTGCG22900.013.06416436
CACCGGT23650.013.02226926
TGCGATA23500.013.01091239
Produced by FastQC (version 0.11.2)