FastQCFastQC Report
Wed 24 May 2017
G153-M11-Index-5_ACAGTG-_ACAYD3ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M11-Index-5_ACAGTG-_ACAYD3ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15007145
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN339650.22632552694066727No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA238430.15887765461052053No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT225280.15011516181125725No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT178500.1189433433207982No Hit
GGCAACTTTAGACTTTTTCTGGAGCAAATCCATGTCTTGGAGAATTCCTT156840.10451021829935007No Hit
AAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAG150630.10037218938045843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC42750.027.1689281
ACGATCA43850.025.8868712
CGATCAC52800.021.7507323
TCACAAG85000.014.0274866
TAATGTA78700.011.8830724
AATGTAG81800.011.7276975
GATCACA106300.011.4473884
TTAGATC19000.010.7678452
CTTTAAT105450.010.4511921
TAATGGG81900.010.38915919
GTAGAAA85800.010.3071418
ATGTAGA95800.010.149776
TTAATGT96750.010.0737253
TAGCCTC51800.09.9801622
GGCATAC85650.09.7598730
TGGCATA87750.09.70185129
TAACCGT10450.09.67911615
CTTAGAT23650.09.5803371
TCTTCGT49850.09.55612544
CACAAGA128850.09.4414827
Produced by FastQC (version 0.11.2)