FastQCFastQC Report
Wed 24 May 2017
G153-M12-Index-6_GCCAAT-_ACAYD3ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M12-Index-6_GCCAAT-_ACAYD3ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14504604
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC298970.20612076000144508No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT279030.1923734008870563No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC237930.16403757041557288No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT186220.1283868211776068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA12800.017.9487131
ACCGGTA18000.016.37676627
TCGGTTT30100.015.7852572
TACGTCA98300.015.3754432
GTCGGTT28200.015.1971641
ATACGTC99850.015.136531
CATACGT105100.014.92391230
TCATACG105650.014.78375329
ACGTCAC103000.014.78063933
CGAATGT23100.014.0934443
CGTCACA108350.014.04931334
CGGGGAT16150.013.9520351
AATTGCG21200.013.90398836
CACCGGT21150.013.83361426
ATTGCGA21000.013.82690737
ATTGGAC102100.013.816
CTCGGTT16850.013.7657321
GCGCATT26300.013.720242544
AATCCGA20100.013.68074624
AAATCCG20500.013.62814523
Produced by FastQC (version 0.11.2)