FastQCFastQC Report
Wed 24 May 2017
G153-M12-Index-6_GCCAAT-_ACAYD3ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M12-Index-6_GCCAAT-_ACAYD3ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14504604
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN317170.21866850001558127No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA198090.13657042963737584No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT185520.12790421579244768No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT170430.11750062256094686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC41600.027.7616021
ACGATCA44950.025.1553342
CGATCAC51200.022.258873
TCACAAG85600.014.0059276
TAATGTA79250.012.4944844
AATGTAG82700.012.1854335
GATCACA101750.012.0671544
CTTTAAT104400.010.91461
CCACGAT19200.010.8843121
TTAATGT94350.010.6798933
ATGTAGA96050.010.6269766
TAATGGG82250.010.58503919
GTAGAAA87450.010.3136678
CTTAGAT24200.010.2716851
CCACTAG24600.010.1956063
GGCATAC87700.010.08356430
TTTAATG104350.09.9294482
TCGTACG2007.643231E-49.89940552
CACAAGA124250.09.8970217
TTAGATC21650.09.8562212
Produced by FastQC (version 0.11.2)