FastQCFastQC Report
Wed 24 May 2017
G153-M13-Index-25_ACTGAT-_ACAYD3ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M13-Index-25_ACTGAT-_ACAYD3ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15410000
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT193400.12550292018170017No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC178690.11595717066839714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGT25350.017.5278383
CGGGATA9950.016.4307021
CTCGAAT31900.015.721091
ACCGGTA18350.014.74572927
ATCCGAA22500.014.17699325
AATCCGA22850.014.05606824
TACGTCA60200.013.59499132
ATACGTC62400.013.256494531
TAAGGCG31500.013.1979859
TCGGTTT31900.013.0324512
AATTGCG21250.012.83639436
CGGGGAT16950.012.7733211
AAATCCG25150.012.77012623
CATACGT64800.012.76468530
TCATACG64850.012.75484329
GTCGGTT31100.012.7156961
ATTGCGA22000.012.69876237
TGCTGCG27250.012.67312911
TCCGCCC34050.012.66298929
ATTGGAC81200.012.6504096
Produced by FastQC (version 0.11.2)