FastQCFastQC Report
Wed 24 May 2017
G153-M13-Index-25_ACTGAT-_ACAYD3ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M13-Index-25_ACTGAT-_ACAYD3ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15410000
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN345820.2244127190136275No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT159470.10348475016223231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC33700.026.045191
ACGATCA35300.023.74422
CGATCAC38250.021.684733
TCACAAG70800.011.9932596
CCACTAG33250.010.7854943
TAATGTA62050.010.6036944
AATGTAG64150.010.4612655
ATCGCTC13850.010.35066742
CGAAAAT33500.010.178211
GATCACA92650.09.9754314
CGCAATT19650.09.9635021
GGCAACT97550.09.9447961
CGACGAT5600.09.8205671
GCATATC26200.09.7395726
ATAGTTA27350.09.64695315
TACCGGT19300.09.460093510
TTAGATC22350.09.4493282
CTTAGAT25700.09.4155161
GCAACTT93300.09.3844472
TAGATCC21950.09.3216453
Produced by FastQC (version 0.11.2)