FastQCFastQC Report
Wed 24 May 2017
G153-M14-Index-27_ATTCCT-_ACAYD3ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M14-Index-27_ATTCCT-_ACAYD3ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15258773
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN337240.22101383905507999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC26850.020.4840621
ACGATCA26700.019.6940142
CGATCAC27200.019.252313
TAATGTA50750.011.0573734
CCACTAG37800.010.1864083
TCACAAG56150.09.9903836
TTATCCG7500.09.9726226
AATGTAG56450.09.9007365
ATTAACG35600.09.51090820
CGAAAAT27600.09.4057461
TTAGATC20700.09.3531922
TTAACGA37300.09.19902121
TAATGGG50950.09.19144419
TAGCCGT10800.09.16036534
GTACGTA4856.366463E-109.07178724
CTTTAAT78600.09.0406651
TAACGAA38050.08.96125322
TAGCCTC60550.08.9022452
CGGAGAG23750.08.8925841
CGAAAAC10000.08.7999531
Produced by FastQC (version 0.11.2)