FastQCFastQC Report
Wed 24 May 2017
G153-M16-Index-8_ACTTGA-_ACAYD3ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M16-Index-8_ACTTGA-_ACAYD3ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17045958
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN385240.2260007915072887No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA331640.1945563869158894No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT310390.18209008845381408No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT249400.14631034524430953No Hit
GGCAACTTTAGACTTTTTCTGGAGCAAATCCATGTCTTGGAGAATTCCTT247020.14491411981655708No Hit
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTT215130.12620587238335326No Hit
AAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAG214840.12603574407492968No Hit
CTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAACCTTCCAGC211380.12400593736063412No Hit
AAAAGATAGAAGATAATGGCAACTTTAGACTTTTTCTGGAGCAAATCCAT198880.11667282061823689No Hit
AGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTG191350.1122553510926168No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC181610.10654138652694087No Hit
GGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGT176790.10371373671107251No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC62900.028.8517861
ACGATCA65550.027.352152
CGATCAC78500.022.7296963
TCACAAG117350.015.5401866
GATCACA137250.013.7076874
CCACGAT25000.012.7584351
TAATGTA103750.012.08919054
CGACGAT4950.011.9985281
AATGTAG108000.011.7953645
CACAAGA160050.011.531647
ATTAACG99700.010.4525120
TAATGGG106600.010.43585819
GTAGAAA110550.010.426958
TATACGC1904.7692467E-410.4201437
ATGTAGA124100.010.4052156
TTAACGA101050.010.40437721
ATCACAA189700.010.4035535
CTTTAAT131550.010.4008511
TAACGAA101200.010.36856822
CGACAAT12350.010.3307151
Produced by FastQC (version 0.11.2)