FastQCFastQC Report
Wed 24 May 2017
G153-M2-Index-19_GTGAAA-_ACAYD3ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M2-Index-19_GTGAAA-_ACAYD3ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15387629
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGT22650.016.4126853
AATCCGA19650.015.78515224
CTCGAAT28100.015.56920051
TCGGTTT30000.015.4710912
ATCCGAA20750.015.26644825
GTCGGTT29250.014.4282931
AAATCCG21550.014.39309423
GCGCATT40050.013.67903344
CGGGGAT18650.013.6246841
CTCGTTT23550.013.5107371
TTCCGCC42150.013.1520628
GTTCCGC42150.013.04767827
ACGTGCC23700.012.99326420
TCCGCCC42200.012.98008929
CCGCCCT42900.012.9221730
CTCGGTT19500.012.9174781
AGTTCCG43000.012.84083526
CGCCCTG44250.012.52862631
CGGTTTG33550.012.5228163
CTCGTAT8650.012.5166311
Produced by FastQC (version 0.11.2)