FastQCFastQC Report
Wed 24 May 2017
G153-M2-Index-19_GTGAAA-_ACAYD3ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M2-Index-19_GTGAAA-_ACAYD3ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15387629
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN352520.22909312409338695No Hit
CTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTCTTCGT157230.10217948457166467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC18200.018.2534473
CACGATC20700.017.4281671
ACGATCA22650.014.8607662
CGTATGC13650.014.0205431
TAGCCTC75550.011.0654012
CCACTAG42200.011.0004223
CTTAGAT26100.010.535321
CTAGCCT86300.010.0684951
TAATGTA33800.010.0261514
TACGCAA9750.09.9272229
CGACAAT9600.09.8531591
ACGCTAA8150.09.71714726
ATAGTTA25600.09.53375315
TCTTCGT82000.09.4946344
GCATATC26350.09.4338786
CTCTTCG83450.09.3558743
CGCAATT19350.09.3220431
TTAGATC23750.09.2630592
GGGTAAC82800.09.24594523
AATGTAG40900.09.1455325
Produced by FastQC (version 0.11.2)