FastQCFastQC Report
Wed 24 May 2017
G153-M3-Index-20_GTGGCC-_ACAYD3ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M3-Index-20_GTGGCC-_ACAYD3ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16186832
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGT24950.014.5465453
TACGTCA26550.014.41893432
ACCGGTA19950.014.22513327
CTCGAAT30800.013.7038771
AATCCGA23300.013.69044524
AAATCCG24000.013.47421623
ATCCGAA23650.013.39490125
AATTGCG21550.013.3721136
ATACGTC28250.013.23939631
TCCGCCC39450.013.10479929
TCATACG28100.013.07433729
GCGCATT40900.013.01776544
AGTTCCG40100.012.89233726
GTTCCGC41250.012.85294327
GTCGGTT32700.012.77246951
TCGGTTT33400.012.7103432
TAAGGCG41150.012.6151959
CGGGGAA25600.012.6029931
CGCCCTG42400.012.55703731
ATCATGC32350.012.4431017
Produced by FastQC (version 0.11.2)