FastQCFastQC Report
Wed 24 May 2017
G153-M3-Index-20_GTGGCC-_ACAYD3ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M3-Index-20_GTGGCC-_ACAYD3ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16186832
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN352940.2180414302193289No Hit
CTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTCTTCGT164300.10150225813179503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC18850.017.1591153
CACGATC22250.016.017831
ACGATCA22900.014.6038472
CCACTAG46350.012.200353
CGAAAAT32300.011.2382861
TAATGTA34900.010.3412924
AATGTAG37250.010.3371145
CGCAATT19200.010.3124041
CACTAGC54750.010.2095364
TAGCCTC83700.09.9100322
CGCTTAT3604.2631655E-89.7776871
TTAGATC24100.09.76845552
CGATACA27650.09.73089244
CTAGCCT93300.09.47901251
CACGATA27950.09.46807642
CCGGAAT36350.09.4414531
ACTAGCC59600.09.3772395
CGGAATT32450.09.2889162
CGACAAT11950.09.2049361
TAGATCC23250.09.17989353
Produced by FastQC (version 0.11.2)