FastQCFastQC Report
Wed 24 May 2017
G153-M4-Index-1_ATCACG-_ACAYD3ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M4-Index-1_ATCACG-_ACAYD3ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14867384
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN335190.22545324718861096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC19900.018.6809081
CGATCAC19100.018.1996523
ACGATCA21400.016.4477832
CGTATGC14000.016.3406621
CCACTAG33200.011.9281673
ACGCAAC8600.011.254683510
TTAGATC20050.010.3137032
TAGCCTC63900.010.2936982
TATGCCG14850.010.2226143
CGACAAT9050.010.2085741
CGTATTA9950.010.16983310
GCATATC24300.010.1393126
ATTAACG21600.09.975347520
TACGCAA10150.09.752889
CCGTTAA10650.09.70295318
TCTTCGT64350.09.69915544
CTAGCCT73900.09.6441721
CACTAGC43400.09.4814554
ATAGTTA25550.09.46609115
TAATGTA34250.09.3160234
Produced by FastQC (version 0.11.2)