FastQCFastQC Report
Wed 24 May 2017
G153-M6-Index-21_GTTTCG-_ACAYD3ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M6-Index-21_GTTTCG-_ACAYD3ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21897865
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT44150.016.2136651
CGAATGT35500.016.172413
TAAGGCG68400.013.9253549
GTCGGTT35400.013.6680881
TCCGCCC64500.013.57421329
AACGTGC36550.013.54028819
ACCGGTA24700.013.53751427
ACGTGCC37250.013.52203320
AGTTCCG64750.013.41984826
TCGGTTT39350.013.4161552
GCGCATT65500.013.368896544
GTTCCGC65450.013.30999327
CCGCCCT67650.012.97473130
TTCCGCC67800.012.9135528
CTCGGTT28050.012.83871651
TACGTCA22200.012.685245532
CTCGTTT35450.012.6516351
CGCCCTG69500.012.63060131
AATTGCG27000.012.13972536
AGGCGTC79800.012.07315711
Produced by FastQC (version 0.11.2)