FastQCFastQC Report
Wed 24 May 2017
G153-M8-Index-4_TGACCA-_ACAYD3ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M8-Index-4_TGACCA-_ACAYD3ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15817806
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN345450.21839311975377623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC16300.017.5470523
CACGATC19500.015.5673811
ACGATCA20600.013.6698442
CCACTAG38900.010.8026973
CGCGTAT2151.306497E-410.2313431
TAGCCTC70000.09.9313862
CGACGAT4959.094947E-129.7766161
CGTATGC12450.09.5410361
TCTTCGT69350.09.44504744
TCATACG31850.09.32394210
CTAGCCT82300.09.2747181
ACGCAAC9300.09.22479810
CGAAAAT25700.09.1584441
TACGCAA10850.08.9208269
ATACGAT33750.08.86165212
GGAAAAC108250.08.8192761
CTCTTCG75450.08.73978243
CGCAATT17600.08.6239751
CGACAAT9950.08.6220911
CTTAGAT26000.08.5451391
Produced by FastQC (version 0.11.2)