FastQCFastQC Report
Wed 24 May 2017
G153-M9-Index-22_CGTACG-_ACAYD3ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M9-Index-22_CGTACG-_ACAYD3ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15350237
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGT25200.017.0211223
TCGGTTT31500.015.4324342
GTCGGTT31400.015.19910051
CTCGAAT30550.015.1880451
ACCGGTA21300.014.97601627
CGGGGAT15700.014.9176351
CGGGATA8950.014.5654111
TACGTCA43050.014.25819132
CGGTTTG31450.014.0581943
AATTGCG22600.014.01594336
ATTGCGA22800.013.89299637
ATACGTC45400.013.61676531
CACCGGT24150.013.57303626
CATACGT46450.013.54533130
TCATACG46400.013.4176929
TTGCGAT24550.013.35067138
TAAGGCG35800.013.1490629
TGCGATA24900.012.98573339
CGCGTTT3253.6379788E-1212.917071
AATCCGA20450.012.90905424
Produced by FastQC (version 0.11.2)