FastQCFastQC Report
Wed 24 May 2017
G153-M9-Index-22_CGTACG-_ACAYD3ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG153-M9-Index-22_CGTACG-_ACAYD3ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15350237
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN347800.2265763062811343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC27650.022.0378911
ACGATCA27850.020.775572
CGATCAC27900.020.5031913
TTAGATC22400.011.2946372
ATCGCTC14300.011.1058642
TAATGTA44650.011.0889834
AATGTAG50700.010.3724685
TAGATCC23400.010.3425863
CGACAAT10100.010.2367461
TCACAAG58100.010.2229416
CGACGAT5450.010.0908771
CCACTAG35550.09.6546683
CGCAATT19900.09.6172651
CTTAGAT27250.09.5257881
TATACGC2403.9492262E-49.165947
GGAAAAC104250.09.1368651
TAGCCTC62450.09.1240942
CCGGAAT36650.08.9432981
TAATGGG47550.08.87739719
GACGATC9500.08.7999942
Produced by FastQC (version 0.11.2)