FastQCFastQC Report
Tue 27 Jun 2017
G154-M1-Index1_ATCACG-_ACB6A7ANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M1-Index1_ATCACG-_ACB6A7ANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19306510
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA4950.010.2237961
CTCGTAT5601.200533E-108.6428942
CGTATGC5903.4924597E-108.20325144
ATCGGAA7650.08.053572
GATCTCG7601.8189894E-117.526539
CGATCTC7406.202754E-107.135309738
TCTCGTA6653.0207957E-86.947484541
CACGATC8351.70985E-106.85046736
ACGATCT8703.0486262E-96.32168337
TCGTATG7452.604429E-76.201236743
ATCTCGT10356.330083E-105.95180640
CGTCTGA8953.677087E-85.899188516
ACACGTC12151.6734703E-105.61284113
TCGGAAG10104.571375E-75.2282033
Produced by FastQC (version 0.11.2)