FastQCFastQC Report
Tue 27 Jun 2017
G154-M1-Index1_ATCACG-_ACB6A7ANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M1-Index1_ATCACG-_ACB6A7ANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19306510
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2900.015.93091110
CGTCGTG3350.013.79065112
GCGTCGT3800.012.73664411
GATCGGA4501.8189894E-1210.763481
GTCGCCG3603.8508006E-910.38964444
GAGCGTC5803.6379788E-129.1034969
GTCGTGT7305.820766E-117.533972713
ATCGGAA7201.9053914E-86.7273332
AGAGCGT7608.054485E-96.65844738
TCGTGTA7952.935849E-96.641268314
TGGTCGC5401.9471827E-56.518991542
CGTGTAG7758.30114E-86.244675615
GTGGTCG6403.6584534E-55.844083341
GGTCGCC6758.214157E-55.541142543
AAGAGCG9601.5951446E-75.50061277
TCGGAAG10755.406764E-85.3249753
Produced by FastQC (version 0.11.2)