FastQCFastQC Report
Tue 27 Jun 2017
G154-M2-Index2_CGATGT-_ACB6A7ANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M2-Index2_CGATGT-_ACB6A7ANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21934742
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA6700.016.7478491
CGTATGC7500.014.96004944
CGATGTA7800.014.10257734
ACCGATG8300.013.7830232
CTCGTAT8250.013.60038542
CACCGAT8550.013.12222131
TCGTATG8800.013.00004243
CCGATGT8750.012.8228433
ATCGGAA9250.012.6063952
TCTCGTA8750.012.32046141
GTCACCG10400.011.63420329
CGTCTGA10750.011.05087116
ACGTCTG13250.09.62995615
TCGGAAG13650.08.8647593
TATCTCG13300.08.60192339
ATCTCGT15100.07.72216140
ACACGTC15000.07.626413313
CGGAAGA16600.07.0240874
TCACCGA20150.06.22301930
GCACACG24550.05.01812911
Produced by FastQC (version 0.11.2)