FastQCFastQC Report
Tue 27 Jun 2017
G154-M2-Index2_CGATGT-_ACB6A7ANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M2-Index2_CGATGT-_ACB6A7ANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21934742
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT4150.023.85539811
AGCGTCG4350.023.77019910
CGTCGTG4750.020.84146512
GATCGGA7850.014.3036181
GTCGCCG4400.013.00069844
GAGCGTC8300.012.7229659
GTCGTGT8950.011.79846613
ATCGGAA9500.011.12443352
TCGTGTA9850.010.72040914
CGTGTAG10700.09.86842815
GCGACGC1900.00553151649.2698335
AGAGCGT12300.09.1225668
GGTCGCC7100.08.98639943
TGGTCGC6700.08.86612842
TCGGAAG13300.08.6081923
GATCTCG8450.08.32888134
AAGAGCG13700.08.0299957
GTGGTCG8101.0913936E-117.333610541
CGGAAGA16300.07.2934954
TCTCGGT11453.45608E-115.954803536
Produced by FastQC (version 0.11.2)