FastQCFastQC Report
Tue 27 Jun 2017
G154-M4-Index4_TGACCA-_ACB6A7ANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M4-Index4_TGACCA-_ACB6A7ANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19689967
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA5700.013.5104191
CGTATGC6450.011.93825744
CTCGTAT6650.010.91768242
TCTCGTA7550.09.61640741
TCGTATG7500.09.38688143
CGTCTGA9300.08.75237716
ATCGGAA8600.08.6985582
ACGTCTG10250.07.726515315
TCGGAAG12000.06.78364323
AATCTCG11500.06.504661639
ACACGTC12900.06.30978913
ATCTCGT12650.06.087311740
CGGAAGA13650.05.963344
Produced by FastQC (version 0.11.2)