FastQCFastQC Report
Tue 27 Jun 2017
G154-M4-Index4_TGACCA-_ACB6A7ANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M4-Index4_TGACCA-_ACB6A7ANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19689967
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG3600.020.16599512
AGCGTCG3850.018.8573310
GCGTCGT4100.018.24380511
GATCGGA5200.013.1250231
GTCGCCG3451.9099389E-910.84112844
TCGTGTA7600.010.42065314
GTCGTGT7250.010.0134113
GAGCGTC7750.09.9356339
ATCGGAA7650.09.7853622
CGTGTAG8150.09.17728215
TCGGTGG7501.8189894E-127.919313438
TGGTCGC5505.695256E-87.600384742
AGAGCGT10500.07.5436038
TCGGAAG10650.07.44238763
GTGGTCG6003.378227E-87.333499441
AAGAGCG10650.07.02427637
GGTCGCC7158.174866E-76.154157643
CGGAAGA13151.8189894E-125.8595484
CGGTGGT8553.3555116E-65.403177739
GATCTCG7901.6051029E-45.011230534
Produced by FastQC (version 0.11.2)