FastQCFastQC Report
Tue 27 Jun 2017
G154-M5-Index5_ACAGTG-_ACB6A7ANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M5-Index5_ACAGTG-_ACB6A7ANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20213819
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA5750.010.7140031
CTCGTAT5650.010.51385142
CGTATGC6100.010.098515544
TCGTATG6650.09.59413143
TCTCGTA6151.8189894E-128.94366941
ATCGGAA7650.08.628012
CGTCTGA9600.07.333191416
GATCTCG9001.8189894E-127.08945339
TCGGAAG12100.06.18194343
ACGTCTG11252.1827873E-116.062090415
ATCTCGT11007.4578566E-116.000388640
ACACGTC11851.4551915E-115.940725313
CGGAAGA14951.8189894E-125.44464354
Produced by FastQC (version 0.11.2)