FastQCFastQC Report
Tue 27 Jun 2017
G154-M5-Index5_ACAGTG-_ACB6A7ANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M5-Index5_ACAGTG-_ACB6A7ANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20213819
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG3450.018.4924212
AGCGTCG4000.015.40025610
GCGTCGT4250.015.0119411
GTCGCCG4203.6379788E-1211.000401544
GATCGGA6100.010.1064881
ATCGGAA7150.08.9305232
GAGCGTC7700.08.8573129
GTCGTGT7600.08.68396913
TGGTCGC5802.4556357E-108.34509142
TCGTGTA8800.08.24974914
GTGGTCG7255.456968E-127.88978741
CGTGTAG9650.07.52277115
GGTCGCC7051.6862032E-97.177566543
AGAGCGT11450.06.91771138
TCGGAAG11303.6379788E-126.2352883
AAGAGCG12601.8189894E-125.9371957
GATCTCG8056.7381297E-65.46395434
CGGAAGA14501.200533E-105.1624784
TCTCGGT9504.2271513E-65.09324536
Produced by FastQC (version 0.11.2)