FastQCFastQC Report
Tue 27 Jun 2017
G154-M6-Index6_GCCAAT-_ACB6A7ANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M6-Index6_GCCAAT-_ACB6A7ANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20030299
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA4153.6379788E-1211.1340131
CGTATGC5900.09.69473144
TCTCGTA6401.8208084E-97.562601641
CGCCAAT6452.1318556E-97.503957733
CTCGTAT6452.1318556E-97.50382642
TCGTATG7253.0304363E-96.97917743
ATCGGAA7856.908722E-75.8860022
ACGTCTG11002.7885108E-95.59980915
ACGCCAA8657.0299575E-75.59532532
GTCACGC8707.856743E-75.563098429
CGTCTGA9901.698214E-65.11093716
TCGGAAG11706.735172E-85.0772573
Produced by FastQC (version 0.11.2)