FastQCFastQC Report
Tue 27 Jun 2017
G154-M7-Index7_CAGATC-_ACB6A7ANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M7-Index7_CAGATC-_ACB6A7ANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20178859
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA6350.011.78084851
ATCGGAA7150.011.0779182
TCGTATG8100.010.86404243
CGTATGC7600.010.42090444
CTCGTAT7450.010.04029842
TCTCGTA7350.09.27912341
CGTCTGA9650.08.43496616
ACGTCTG10600.08.30154115
ATCTCGT10250.07.083070840
TCGGAAG12300.06.6181033
CGGAAGA12450.06.36144924
ACACGTC12700.06.062695513
CATCTCG12303.8198777E-115.723821639
Produced by FastQC (version 0.11.2)