FastQCFastQC Report
Tue 27 Jun 2017
G154-M7-Index7_CAGATC-_ACB6A7ANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M7-Index7_CAGATC-_ACB6A7ANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20178859
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3750.020.53352210
CGTCGTG4000.018.6994312
GCGTCGT4050.018.46921511
GTCGCCG3650.013.2613544
GATCGGA5300.012.8773061
GAGCGTC6600.011.6668039
TCGTGTA8000.010.4496314
CGTGTAG9750.09.02500815
ATCGGAA7900.08.9180892
GTGGTCG6251.4551915E-118.4485641
AGAGCGT9700.08.3922888
GTCGTGT9450.07.91506413
TGGTCGC6452.582965E-107.845597742
GATCTCG9153.092282E-116.73013234
GGTCGCC8205.697075E-96.43954743
AAGAGCG11350.06.39702657
TCGGAAG12205.456968E-125.95529133
CGGAAGA13751.4733814E-105.2835094
Produced by FastQC (version 0.11.2)