FastQCFastQC Report
Tue 27 Jun 2017
G154-M8-Index8_ACTTGA-_ACB6A7ANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M8-Index8_ACTTGA-_ACB6A7ANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19573679
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA5950.015.5307131
TCTCGTA6350.012.81915541
TCGTATG7700.010.85708243
CGTATGC7500.010.85327344
CTCGTAT7800.010.71802642
CGTCTGA9800.09.87724316
ATCGGAA9050.09.7244472
ATCTCGT10300.09.18460340
AATCTCG11050.07.764980339
TCGGAAG12450.07.4218733
ACGTCTG12700.07.102123315
CGGAAGA14800.06.9865364
ACACGTC13200.06.83303313
GCACACG22600.05.353774511
Produced by FastQC (version 0.11.2)