FastQCFastQC Report
Tue 27 Jun 2017
G154-M8-Index8_ACTTGA-_ACB6A7ANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M8-Index8_ACTTGA-_ACB6A7ANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19573679
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT4050.020.6416311
CGTCGTG3850.020.57024212
AGCGTCG4550.018.37345910
GTCGCCG3850.013.71521444
GATCGGA6750.013.0464261
GAGCGTC7900.011.1391699
CGTGTAG8500.010.61088215
GTCGTGT8350.010.53826113
ATCGGAA8200.010.4715132
TCGTGTA8450.010.15320914
TGGTCGC6151.8189894E-128.94364842
AGAGCGT9750.08.3494328
TCGGAAG11450.07.6915383
GTGGTCG7501.2732926E-117.62704641
AAGAGCG12100.07.4553357
GATCTCG9200.07.410967434
GGTCGCC7601.1059456E-96.947820743
TCTCGGT10950.06.829381536
CGGAAGA14750.06.2687584
TCGGTGG10855.0931703E-116.082489538
Produced by FastQC (version 0.11.2)